Abstract: Based on the theory of refined management of enterprise, combining with the exploration of practical teaching management of the gemmological laboratory teaching in the Art and Communication College of Wuhan University of Engineering Science, this paper summarizes the core connotation of gemmological laboratory management, and refines the management elements of "safety, profession, standard, order, efficiency and responsibility". The detailed exploration of management work is carried out in five aspects: institutionalization of management work, professionalization of management personnel, flow of management process, standardization of experimental operation and concretization of responsibility implementation. Through analyzing the effect of the implementation of refined management, the authors share the management experience of gemmological laboratory, so as to provide reference for promoting the transformation of gemmological professional laboratory in application-oriented colleges from traditional extensive management to standardized and refined management.